Journal: bioRxiv

Article Title: Defining the in vivo mechanism of air pollutant toxicity using murine stress response biomarkers

doi: 10.1101/2022.10.05.510981

Figure Lengend Snippet: A . Triplicate HOTThet reporter mice were instilled with PM 10 from Marylebone St. and LacZ staining was performed in lungs (upper panels). Cryosections derived from the same samples were subjected to F4/80 immunohistochemistry (lower panels). B . Effect of 10 μg/mL Marylebone Road PM10 (MR-PM10) on PAFR expression in human primary nasal epithelial cells. Data are from 5 separate experiments and summarised as median fluorescent intensity (MFI). Figure represents the median value and p values are calculated by Mann Whitney test. C . Effect of 10 μg/mL Marylebone Road PM10 (MR-PM 10 ) on pneumococcal adhesion to human primary nasal epithelial cells (HPNEpC) and PAFR blocker CV3988 on MR-PM 10 stimulated pneumococcal adhesion to HPNEpC. Data are from 6 separate experiments. Data are summarised as median IQR Kruskal– Wallis with post hoc multiple comparison testing. D . Effect of anti-oxidant N-acetylcysteine on MR-PM10 stimulated pneumococcal adhesion to HPNEpC. Data are from 7 separate experiments and are summarised as median IQR Kruskal–Wallis with post hoc multiple comparison testing. Black arrow indicates reporter activation.

Article Snippet: Human primary nasal epithelial cells (HPNEpC) were purchased from PromoCell® (Heidelberg, Germany), and maintained in airway epithelial cell growth medium, with supplement kit, Primocin (InvivoGen, San Diego, USA), and 10% FBS.

Techniques: Staining, Derivative Assay, Immunohistochemistry, Expressing, MANN-WHITNEY, Activation Assay

Journal: The Journal of Allergy and Clinical Immunology: Global

Article Title: Curbside particulate matter and susceptibility to SARS–CoV-2 infection

doi: 10.1016/j.jacig.2023.100141

Figure Lengend Snippet: Effect of PM 10 on ACE2 expression. PM 10 was collected from the curbside of Marylebone Road (curbside PM 10 ) and the exhaust of a diesel car (diesel PM 10 ) by using the same cyclone. Cells were cultured with PM 10 for 2 hours, and ACE2 expression was determined by flow cytometry. The results are expressed as mean fluorescence intensity (MFI) adjusted for isotypic antibody control. A, Dose-response relationship between curbside PM 10 and ACE2 expression by A549 cells. Two data points are omitted for visual convenience. B , Replication of A549 dose-response data using 10 μg/mL of curbside PM 10 . C , Effect of curbside PM 10 and diesel PM 10 (10 μg/mL) on ACE2 expression (MFI) in purchased (Promocell) HPNEpCs. D, Effect of curbside PM 10 (10 μg/mL) on ACE2 expression in primary nasal epithelial cells obtained from a local adult donor HPNEpCs. E, Effect of curbside PM 10 (10 μg/mL) on ACE2 expression (MFI) in Calu3 cells. F, Effect of the antioxidant N-acetyl cysteine on expression of curbside PM 10 -stimulated ACE2 expression (MFI) in purchased HPNEpCs. Columns represent medians from at least 4 separate experiments. Data were compared by either the Mann-Whitney U test or Kruskal-Wallis test and Dunn multiple comparisons test.

Article Snippet: Human primary nasal epithelial cells (HPNEpCs) were either obtained from a nonsmoking, nonvaping, healthy adult donor by using a dental brush (donor HPNEpCs) or purchased from PromoCell (Heidelberg, Germany).

Techniques: Expressing, Cell Culture, Flow Cytometry, Fluorescence, Control, MANN-WHITNEY

Journal: The Journal of Allergy and Clinical Immunology: Global

Article Title: Curbside particulate matter and susceptibility to SARS–CoV-2 infection

doi: 10.1016/j.jacig.2023.100141

Figure Lengend Snippet: Effect of 5% CSE on ACE2 expression in A549 cells ( A ) and purchased (from Promocell) HPNEpCs ( B ). Results are expressed as mean fluorescence intensity (MFI) adjusted for isotypic antibody control. Columns represent medians from at least 4 separate experiments, and data are compared by the Mann-Whitney U test.

Article Snippet: Human primary nasal epithelial cells (HPNEpCs) were either obtained from a nonsmoking, nonvaping, healthy adult donor by using a dental brush (donor HPNEpCs) or purchased from PromoCell (Heidelberg, Germany).

Techniques: Expressing, Fluorescence, Control, MANN-WHITNEY

Journal: eBioMedicine

Article Title: Underground railway particulate matter and susceptibility to pneumococcal infection

doi: 10.1016/j.ebiom.2022.104063

Figure Lengend Snippet: Effect of 20 µg/mL London Underground particulate matter less than 10 microns from microns from Bakerloo (B-PM 10 ) and Jubilee lines (J-PM 10 ) on human primary epithelial cells. Pneumococcal adhesion to (a) human primary nasal epithelial cells (HPNEpC B-PM 10 and J-PM 10 n=5, technical replicates (TR)=3, p=0.007 and p=0.032, respectively, and (b) primary bronchial epithelial cells (HPBEpC; B-PM 10 and J-PM 10 n=5, TR=3, p=0.017 and p=0.014, respectively). Platelet-activating factor receptor (PAFR) median fluorescence intensity (MFI) expression, (c) HPNEpC (B-PM 10 and J-PM 10 , n=5, TR=2, p= 0.011 and p=0.02, respectively) and, (d) HPBEpC (B-PM 10 and J-PM 10 , n=5, TR=2, p=0.013 and p=0.003, respectively. Columns represents median and p values are calculated by Kruskal-Wallis with post hoc multiple comparison testing.

Article Snippet: Human primary nasal epithelial cells (HPNEpC), and human primary bronchial epithelial cells (HPBEpC) and were purchased from PromoCell® (Heidelberg, Germany), and maintained in airway epithelial cell growth medium, with supplement kit, Primocin (InvivoGen, San Diego, USA), and 10% FBS.

Techniques: Fluorescence, Expressing